What compounds or probes could you use to investigate (a) G-protein–mediated signal transduction, (b) nitric oxide signal transduction, and (c) calcium ion-dependent fluxes and effects
What will be an ideal response?
Answer:
a) Nonhydrolyzable GTP analogues prolong G-protein–mediated processes because the G-protein cannot turn itself off.
b) Nitroglycerin releases NO, which can stimulate NO-dependent processes in cells.
c) Calcium ion fluxes can be measured and visualized using sensors such as cameleon that fluoresce when binding calcium ions. Calcium ion levels can be increased in cells using a calcium ionophore, which transports calcium ions across membranes.
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One goal of pedigree analysis is to determine ____
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a. It is joined by an ionic bond only. b. It is always in equilibrium with the reactants. c. It is the substance that initiates the reaction. d. It is generally written on the left side of the equation. e. It is generally written on the right side and is the substance generated by the reaction.