Which of the following would you include when performing a Gram stain?
A. Rub the slide to hasten drying
B. Allow the stain to sit for no more than 10 seconds before rinsing
C. Cover only a small area of the specimen on the side with the crystal violet stain
D. Cover the entire specimen area with iodine and allow it to remain for 1 minute
E. Apply exactly 3 drops of alcohol or decolorizer after rinsing away the iodine
Answer: D
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