A procedure using both reverse transcriptase and real time PCR can be used to
A) determine the number of copies of a DNA sequence in a sample.
B) determine the number of copies of an RNA sequence in a sample.
C) produce DNA fragments for cDNA cloning.
D) both determine the number of copies of an RNA sequence in a sample and produce fragments for cDNA cloning.
E) produce RNA fragments for cloning.
D
Bloom's Taxonomy: Application
Section: Techniques of Recombinant DNA Technology
Learning Outcome: 8.12
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Noncompetitive inhibitors of a reaction will bind _______
A. to the substrate B. to the active site of the enzyme C. somewhere on the enzyme that is not at the active site D. somewhere on the substrate other than at the active site E. to vitamins that accelerate the reaction
Some scientists believe that RNA was the first information storage molecule because ____
a. some viruses use RNA as their information storage molecule b. RNA can be an enzymatic catalyst as well as an information storage molecule c. amino acids cannot form peptide bonds without ribosomes to catalyze their formation d. RNA is a less stable molecule than DNA e. proteins are made of far more amino acid monomers (over 20) than RNA (4)
Aquaporins are
a. channel proteins for water. b. carrier proteins for solutes. c. channel proteins for solutes. d. carrier proteins for water. e. carrier proteins for water and solutes.
Denaturation of proteins may result in all of
the following EXCEPT a. breakage of hydrogen bonds. b. loss of three-dimensional structure. c. removal of R groups from amino acids. d. alteration of enzyme activity. e. endangerment of a cell's life