A public health microbiologist plans to screen a particular bacterial species that was isolated from several patients in an intensive care unit to detect if any of the cells are penicillin-resistant mutants. What is the first step in conducting this experiment?
A. Test the cells to see if they can grow on media to which penicillin has been added.
B. Perform an antibiotic susceptibility test on the cells using antibiotics other than penicillin that are known to be effective.
C. Set up an Ames test to see if any of the isolates are mutagenic.
D. Determine whether or not wild type cells of the same bacterial species are susceptible to penicillin.
Answer: D
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