You have produced antibodies against the GLUT4 glucose transporter and labeled them with a red fluorescent dye. You culture the cells in the absence of insulin and after a 30-minute incubation period, you fix the cells and treat them with the fluorescent antibody. What do you see when you look at the cells in the fluorescence microscope?
a) The green fluorescent label is concentrated in the cytoplasm around membrane vesicles.
b) The cells are uniformly red; the antibodies are equally distributed between the cell cytoplasm and membrane.
c) The red fluorescent label is concentrated on the surface of the cell in the plasma membrane.
d) The red fluorescent label is concentrated in the cytoplasm around membrane vesicles.
Ans: d) The red fluorescent label is concentrated in the cytoplasm around membrane vesicles.
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