Consider the map shown below of a portion of a prokaryotic genome. How might the elements indicated affect the stability and structure of the genome? Assume that IS2 encodes for a transposase that catalyzes conservative transposition
Re-draw the genome map to illustrate your prediction if necessary.
| IS2 |__gene1 gene2 gene3 | IS2 | geneA geneB geneC | IS2 | geneD | IS2 | geneX
What will be an ideal response?
Answer: Answers should indicate that having multiple IS2 elements in the genome could result in the deletion and/or rearrangement of gene order by transposition events. Composite transposons can form if the genes between to insertion sequences move, or transpose, with the insertion sequences. For instance, geneD could be removed from this part of the genome and inserted elsewhere. OR geneABCD could all move together to another part of the genome through transposition. Similarly gene123 could move together, or even gene123 and geneABCD (but not geneX). If conservative transposition is the only possibility then the genes will move from one spot to another in the genome, but if replicative transposition occurred the genes could be copied to another part of the genome.
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The Punnett square is a useful tool for
A) determining the rate of segregation of alleles. B) calculating how many mutations occur during DNA replication. C) determining which genes or traits assort independently during gamete formation. D) predicting the ratios of possible genotypes of a particular combination of alleles. E) predicting the level of crossing over that will occur during meiosis.
The name of the virus that causes common colds and has been used as a vector of genes is ________.
Fill in the blank(s) with the appropriate word(s).
Many trans–acting regulatory elements reside on a separate chromosome than the gene that it regulates. In this case, the regulatory element is _______ relative to the gene
a. unlinked b. linked c. dominant d. recessive e. codominant
Polymerase chain reactions utilize a heat-stable DNA polymerase that was isolated from the bacterium Thermus aquaticus.
Answer the following statement true (T) or false (F)