Which of the following is evidence for the signal hypothesis for targeting of a secretory protein to the rough ER?
a. The protein is larger when synthesized in vitro on free polysomes.
b. The secretory protein ends up in the cytosol when a short sequence is deleted by genetic engineering.
c. A normally cytosolic protein is secreted when a sequence is added to it by genetic engineering.
d. All of the above
Ans: b. the secretory protein ends up in the cytosol when a short sequence is deleted by genetic engineering
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