How is a DNA fragment containing a gene of interest generated for cloning?
a. The gene with a cloning vector is ligated.
b. Agarose gel electrophoresis takes place
c. The polymerase chain reaction (PCR) amplifies the DNA fragment from the genome
d. A DNA polymerase specific for the fragment is used to make copies
e. DNA fingerprinting allows the fragment to be copied from the chromosome.
ANSWER: c
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