A bone marrow aspirate shows copious blue granules with the Prussian blue stain. Select the correct interpretation:
a. Iron overload
b. Iron deficiency
c. Tumor cells present
d. Megakaryocytes increased
ANS: A
The Prussian blue stain is an iron-specific stain (nonheme iron). When iron is in the storage form of hemosiderin, it stains blue. If copious granules are visible, then it suggests iron overload. The granules must be found in macrophage cytoplasm to rule out possible contamination of the slide or stain with iron.
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