You have the target DNA and primers shown below. You add the target DNA and primers, along with dATP, dTTP, dGTP, dCTP and Taq DNA polymerase to a test tube. After 30 cycles of PCR, which of the following is true about the DNA in your test tube?Target DNA: 5' - CCGGATCAATCAATGCCGAATTTCCGTATAGGGCCTAGTAG - 3' 3' - GGCCTAGTTAGTTACGGCTTAAAGGCATATCCCGGATCATC - 5'Primer 1: 5' - GATCAA - 3'Primer 2: 5' - CGGAAA - 3'
A. There will be around a billion DNA molecules and most will look just like the template
5' - CCGGATCAATCAATGCCGAATTTCCGTATAGGGCCTAGTAG - 3'
3' - GGCCTAGTTAGTTACGGCTTAAAGGCATATCCCGGATCATC - 5'.
B. There will be around a billion DNA molecules and most will contain the region that is flanked by the primers
5' - GATCAATCAATGCCGAATTTCCG - 3'
3' - CTAGTTAGTTACGGCTTAAAGGC - 5'.
C. Only the template and primers will be present; since no DNA helicase was added the strands won't separate so no new DNA can be made.
D. There will be around a billion DNA molecules and most will be single stranded, either
5' - CCGGATCAATCAATGCCGAATTTCCGTATAGGGCCTAGTAG - 3' OR
3' - GGCCTAGTTAGTTACGGCTTAAAGGCATATCCCGGATCATC - 5'.
E. There will be around a million DNA molecules of various sizes, depending on where chain termination occurred.
Answer: B
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