Of the following media, which provides the NAD necessary for the growth of Haemophilus spp.?
a. 5% sheep blood agar
b. Brain-heart infusion agar
c. Chocolate agar
d. Nutrient agar
C
Five percent sheep blood agar provides one of the factors, hemin or X factor, necessary for the growth of Haemophilus spp. Most strains will not grow on 5% sheep blood agar because it con-tains hemin but not NAD. Chocolate, however, contains both necessary nutrients.
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