The primary plating medium used to select out Clostridia spp. would be:
a. anaerobic blood agar.
b. laked kanamycin-vancomycin (LKV) blood agar.
c. phenylethyl alcohol agar (PEA).
d. thioglycollate.
C
The primary plating media for Clostridia spp. is PEA, which selects out gram-positive organisms while inhibiting gram-negative bacteria. LKV is selective for gram-negative organisms, particu-larly Prevotella and Porphyromonas spp. Blood agar and thioglycollate are nonselective media.
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