You have the amino acid sequence of a protein and wish to search for the gene encoding this protein in a DNA library. Using this protein sequence, you deduce a particular DNA sequence that can encode this protein

Why is it unwise to use only this DNA sequence you have deduced as the probe for isolating the gene encoding your protein of interest from the DNA library?


Because most amino acids can be encoded by more than one codon, a given sequence of amino acids could be encoded by several different nucleotide sequences. Probes corresponding to all these possible sequences have to be synthesized, to be sure of including the one that corresponds to the actual nucleotide sequence of the gene and thus will hybridize with it.

Biology & Microbiology

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The activation energy for a reaction

a. is less when enzymes are present. b. allows greater interaction of substrate with the active site. c. is needed to begin a reaction. d. puts the substrate at its transition state. e. is all of these.

Biology & Microbiology

Radioactive iodine (131I) is commonly used in medical situations for diagnosing and treating thyroid conditions. The most common isotope of iodine that is not radioactive is 127I

Chemically, what is the difference between 127I and 131I? What do they have in common?

Biology & Microbiology

Who was the first person to apply antiseptic techniques during surgery?

A. Ignatz Semmelweis B. Louis Pasteur C. Robert Hook D. Joseph Lister E. Paul Ehrlich

Biology & Microbiology

Medicines are often administered in pill form. In many cases, the active ingredient of the pill (the drug) is joined to another substance by ________. This forms a(n) ________, which is stable in the dry environment of a pill bottle but dissociates under

the wet conditions of the digestive system to release the drug to the body. A) ionic bonds; salt B) hydrogen bonds; base C) ionic bonds; acid D) covalent bonds; salt

Biology & Microbiology