A specific treponemal serologic test performed by overlaying whole treponemes that are fixed to a slide with serum from patients suspected of having syphilis and using fluorescein-conjugated antihuman antibody reagent to detect specific antitreponemal antibodies is called the _________ test

a. RPR
b. FTA-ABS
c. VDRL
d. TP-PA


B
Specific treponemal serologic tests include the FTA-ABS and the TP-PA tests. The FTA-ABS test is performed by overlaying whole treponemes that are fixed to a slide with serum from pa-tients suspected of having syphilis because of a previous positive VDRL or RPR test. The pa-tient's serum is first absorbed with non–T. pallidum treponemal antigens (sorbent) to reduce nonspecific cross-reactivity. Fluorescein-conjugated antihuman antibody reagent is then applied as a marker for specific antitreponemal antibodies in the patient's serum.

Biology & Microbiology

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