In two of Koch's postulates (#2 and #3), a pure culture of the organism is required. Which of the following would NOT be a possible consequence of using a contaminated culture?
A. You can't necessarily attribute the illness directly to the microbe in question-it may in fact be caused by the contaminating microbe.
B.
There's the possibility that the test animal might be acutely susceptible to the contaminating microbe, but completely resistant to the microbe you suspect causes the illness of interest. As such, when you introduce it into the test animal, it could confuse your final results.
C.
The problem is that one microbe may be toxic to the other. It may have killed all of your suspect microbe in the culture. Therefore, you can't be sure that you're infecting your test animals with the microbe you suspect is causing the illness, or if it's only the second (contaminating) microbe.
D.
Even though there's a contaminating microbe present, so long as the original suspect microbe is also present, the disease should still manifest in test animals. It should also still be recoverable from test animals following infection. As such, there's really no consequence to using a contaminated culture.
D
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Fill in the blank(s) with the appropriate word(s).