Media that can be used for the isolation of Mycoplasma include:

a. Thayer-Martin agar with vitamin K.
b. 5% sheep blood and IsoVitaleX.
c. beef or soybean protein with serum and fresh yeast extract.
d. hemin and nicotina-mide-adenine-dinucleotide (NAD) sup-plements.


C
In general, the medium for Mycoplasma isolation contains a beef or soybean protein with serum, fresh yeast extract, and other factors. Because these organisms grow more slowly than most other bacteria, the media must be selective to prevent the overgrowth of faster-growing organisms that may be present in a clinical sample.

Biology & Microbiology

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Biology & Microbiology