Compare and contrast the streak-plate method of isolation with the pour-plate method of isolation
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The streak-plate and the pour-plate methods of bacterial isolation are both used to produce pure cultures of bacteria from specimens. Both techniques involve the use of agar-based growth media contained in Petri plates. Additionally, both techniques involve the use of dilutions as a means of isolating single cells or groups of cells that then grow into isolated colonies. However, there are several significant differences between the two techniques. The main difference is the way in which the specimen is diluted. In the streak-plate method, the specimen is diluted by use of an inoculating loop that spreads organisms over the surface of the agar. Thus, colonies appear only on the agar surface. In the pour-plate method, however, dilutions of the specimen are made in tubes of broth and then each dilution is added to melted agar, which is then poured into Petri dishes. Thus, microbes are mixed throughout the agar, and colonies will appear both in and on the medium.
Bloom's Taxonomy: Analysis
Section: Culturing Microorganisms
Learning Outcome: 6.9
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The genotypic ratio produced in the last cross is most likely
A secretor (allele seS) is a person who secretes their blood type antigens into body fluids such as saliva. By comparison, a non-secretor (allele seNS) does not. A person's status as a secretor or non-secretor is independent of blood type. Consider the following inheritance pattern of this trait:
A. 3:1
B. 1:2:1
C. 2:1
D. 9:3:3:1
E. 1:1
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Which of the following diseases is not caused by an obligate intracellular pathogen?
A) chlamydial urethritis B) human monocytic ehrlichiosis C) Rocky Mountain spotted fever D) typhoid fever
Contraceptive methods that rely on synthetic hormones
A) destroy fertilized eggs. B) prevent the implantation of a fertilized egg in the uterus. C) prevent ovulation. D) prevent the sperm and egg from meeting. E) kill sperm.
What happens to Glycerol
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