The pKa of TRIS is HEPES is 7.55. What is the pH of a buffer prepared by mixing 200. mL of 0.20 M HEPES in its acid form with 100. mL of 0.20 M HEPES in it basic form?
A) 6.55
B) 7.25
C) 7.85
D) 8.55
B
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