Outline the three stages involved in the transcription of a DNA molecule. What will be an ideal response?


ANSWER: In the first stage of transcription, initiation, RNA polymerase and several regulatory proteins
bind to a specific nucleotide sequence called a promoter that marks the beginning of a gene.
In the second stage, elongation, the strands of DNA unwind further, and RNA polymerase
reads the nucleotide sequence of the template strand. As it moves along, it inserts and links
together complementary RNA nucleotides to form a pre-mRNA molecule. In the third and
final stage, termination, RNA polymerase moves along the DNA template, and eventually
reaches the end of the gene, marked by nucleotides called a termination sequence. There,
RNA polymerase falls off the DNA template strand, the pre-mRNA molecule is released, and
the DNA strands re-form a double helix.

Biology & Microbiology

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