You have decided to repeat Palade’s experiments on the endomembrane system. You think you’ve done everything correctly, but when you observe your final sample under the microscope, you find that the entire endomembrane system contains tiny black dots. When you look at later timepoints you see the same thing. What part of the experiment did you forget?

Question

A. You forgot to add radioactive amino acids.
B. You forgot to add osmium tetraoxide.
C. You forgot to add nonradioactive amino acids.
D. You forgot to add the silver-containing emulsion.
E. You didn’t forget anything, this is what the experiment is supposed to look like.

Answer 1

C. You forgot to add nonradioactive amino acids.