Scientists like to use fluorescent proteins for various types of recombinant DNA procedures. You have a very small amount of the gene for a fluorescent protein. You'd like to make a fluorescent bacterium (!). Which of the following represents the correct sequence of procedures that you would use?
a. Transform the vector into the bacteria. Amplify the gene using PCR. Insert the gene into a plasmid vector.
b. Amplify the gene using PCR. Transform the vector into the bacteria. Insert the gene into a plasmid vector.
c. Amplify the gene using PCR. Insert the gene into a plasmid vector. Transform the vector into the bacteria.
d. Insert the gene into a plasmid vector. Amplify the gene using PCR. Transform the vector into the bacteria.
Answer: c. Amplify the gene using PCR. Insert the gene into a plasmid vector. Transform the vector into the bacteria.
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