The permanent stained smear protocol of the O&P examination:

Question

a. requires freshly passed, unpreserved stool.
b. is designed to allow the detection of pro-tozoan cysts, coccidian oocysts, micro-sporidial spores, and helminth eggs and larvae.
c. is microscopically examined at low, high dry, and oil immersion magnifications.
d. is designed to facilitate the identification of intestinal protozoa.

Answer 1

D
The permanent stained smear is designed to facilitate the identification of intestinal protozoa. Several staining methods are available; the two most common methods are the Wheatley's mod-ification of the Gomori tissue trichrome and the iron-hematoxylin stains. This part of the O&P examination is critical for the confirmation of suspicious objects observed in the wet examination and for the identification of protozoa that might not have been seen in the wet preparation. The permanent stained smear is the most important procedure performed for the identification of intestinal protozoan infections; the permanent stained smears are examined using oil immersion objectives (600 for screening, 1000 for final review of 300 oil immersion fields).