Twenty cells are counted in one of the nine large squares of a hemocytometer. The sample is diluted 1:10. How many cells are present per mL?

a. 200
b. 1000
c. 2000
d. 20,000


ANS: C
Plugging the appropriate numbers into the formula: (20 ´ 10)/(1 mm2 ´ 0. 1 mm) = 2000/mm3 = 2000/mL (= 2.0 ´ 103/mL = 2.0 ´ 109/L).

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