A __________ shows one path of energy and nutrient flow among organisms. Fill in the blank(s) with the appropriate word(s)
food chain
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In E. coli, ? DNA integrates into which site on the bacterial chromosome?
A) ori B) trp C) bio D) gal E) att
You discover a new fluorescent protein that emits photons with a wavelength of 600 nm. Which of the following wavelengths of light might excite the protein?
A) 455 nm B) 590 nm C) 550 nm D) all of the above
Contamination with ________ is a concern because they are highly resistant to killing
A) archaea B) endospores C) pleomorphs D) vegetative cells E) vibrios
Chromatography is frequently used to purify proteins from cellular extracts. There are various strategies that can be used, depending on the tools and reagents available
You are interested in isolating additional proteins that interact with your protein target, but your labmates have used all the purified protein stocks. A. Why would you need purified target protein to do this experiment? B. What other strategies/tools could you use to carry out the affinity chromatography? C. What are the limitations to the method you described in part B that would not be a concern if you could use the purified protein directly?