An E. coli strain contains a mutation in the malate dehydrogenase gene that results in a stop codon at amino acid position 14
From subcultures of this strain, a colony was obtained that was capable of making malate dehydrogenase. Your approach to examining this phenomenon should be to examine
A) the DNA sequence to see if a reversion has occurred.
B) the DNA sequence to see if a suppressor mutation has occurred.
C) other proteins to see if other stop codons are ignored, indicating the presence of a suppressor tRNA.
D) the DNA and plasmids for the incorporation of a second complete copy of the malate dehydrogenase gene.
E) All of these approaches need to be considered.
Answer: E
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