Which of the following statements about DNA libraries is true?
(a) Production of a DNA library involves the direct insertion of short DNA fragments into bacteria through transformation.
(b) By placing the library DNA into bacteria, the bacteria can be used to amplify the desired DNA fragments from the DNA library.
(c) Individual bacteria that have taken up most of the library DNA are selected for during the construction of a DNA library.
(d) The library DNA within the bacteria will only be replicated when it hybridizes to a DNA probe.
Choice (b) is correct. To make a DNA library, the fragmented DNA must first be placed into an appropriate plasmid before it can be placed into the bacteria through transformation [choice (a)]. Individual bacteria in a library should only carry a single DNA fragment from the library [choice (c)]. Although DNA probes are used to identify the bacterial colony that contains the DNA library fragment of interest, this hybridization is done on dead bacteria and is not involved in replication of the desired DNA [choice (d)].
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