How can foreign DNA of greater than 300 kbp be inserted and stably maintained in BAC vectors?
What will be an ideal response?
Answer: The BAC vector itself is quite small (6.7 kbp), containing minimal genes such as oriS and repE for replication in a host as well as sopA and sopB to minimize the copy number. The host strain should also lack modification and restriction systems to prevent the vector from being digested. A recombination defective host is also important to prevent recombination of the host's chromosome with the BAC vector.
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a. mesoderm. b. epiderm. c. mesoglea. d. endoderm. e. ectoderm.
In the mating of an Aa female and an Aa male, the genotypic outcome predicted by a Punnett square is ________
A) three-quarters Aa and one-quarter AA B) one-half AA and one-half aa C) three-quarters Aa and one-quarter aa D) one-quarter AA, one-quarter aa, and one-half Aa E) All offspring will have the genotype Aa
During which process does the shedding of the endometrium occur?
A) menstruation B) intercourse C) ovulation D) fertilization
Which of the following might an ecologist study?
A) general nature of digestive enzymes of deer B) genetics of mosquitoes C) function of the immune system in human populations D) effects of increasing carbon dioxide on forest growth