You attempt deletion mapping of a part of the promoter region of a particular gene. You remove a short sequence of nucleotides. Once the altered DNA is transfected into cells, the cells are able to transcribe the transfected DNA in a normal fashion. What do you conclude?
a) The deleted sequence has a moderate level of importance in promoting transcription.
b) The sequence that was removed is not an essential part of the promoter.
c) The sequence that was removed is an essential part of the promoter.
d) The sequence that was deleted is an important determinant of the ability to transcribe the gene.
Ans: b) The sequence that was removed is not an essential part of the promoter.
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