Which of the following limits the use of PCR to detect and isolate genes?
(a) The sequence at the beginning and end of the DNA to be amplified must be known.
(b) It also produces large numbers of copies of sequences beyond the 5? or 3? end of the desired sequence.
(c) It cannot be used to amplify cDNAs or mRNAs.
(d) It will amplify only sequences present in multiple copies in the DNA sample.
(a) To construct primers that will bracket the desired gene, you have to know the sequence at the beginning and end of the DNA to be copied.
You might also like to view...
The colinearity rule describes
A. the spatial arrangement of homeotic genes along the chromosome. B. the timing of expression of homeotic genes. C. the long stretches of DNA between homeotic gene complexes. D. the parallel arrangement of homeotic genes between different chromosomes. E. the spatial arrangement of alpha helices in proteins encoded by homeotic genes.
Following reports that stained chromosomes "disappeared" while observing cell division, scientists in the early 1900s determined the molecule of heredity to be
A) DNA. B) RNA. C) protein. D) lipids. E) carbohydrates.
Prokaryotes
a. have nucleoid regions. b. are unicellular. c. may have cell walls. d. are either bacteria or archaeans. e. are all of these.
Living cells maintain biochemical reactions far from equilibrium conditions. They do this by constantly manipulating
the concentrations of reactants and products. Why is it important that a state far from equilibrium be maintained? What will be an ideal response?