If multiple fragments from a genome have been cloned into bacteria, how can a researcher identify the

bacteria that contains the particular sequence they are interested in?



a. The bacteria containing the gene of interest are antibiotic resistant and unable to digest Xgal.
b. The DNA purified from individual colonies of bacteria is probed with a short, single
stranded DNA fragment that is complementary to the gene of interest.
c. The scientists have to sequence all of the DNA from all of the white bacterial colonies;
fortunately computers let us automate this process.
d. The scientists have to sequence all of the DNA from all of the blue bacterial colonies;
fortunately computers let us automate this process.
e. We have to isolate the DNA of interest before transforming it into the bacteria. That is the
only way to know which bacteria have the DNA of interest.


B

Biology & Microbiology

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