You have determined the base sequence for a gene from Felis catus (domestic cat) that you believe may be involved in feline leukemia
The nucleotide and probable amino acid sequences are similar to retroviral proteins that become embedded in the host cell membrane. You separate the membrane fraction of feline leukemia cell lysate from the cytoplasmic fraction. What technique could you use to determine if your protein of interest is present in the membrane fraction?
A) affinity chromatography
B) liquid chromatography followed by mass spectrometry
C) Southern blot analysis
D) two-dimensional gel electrophoresis
E) liquid chromatography followed by mass spectrometry or two-dimensional gel electrophoresis
Answer: E
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A(n) ________ is an instrument used to separate subcellular structures and macromolecules on the basis of size, shape, and density. ________ developed this instrument in Sweden during the period 1925—1930.
Fill in the blank(s) with the appropriate word(s).
At which level of protein structure are peptide bonds most important?
a. primary b. secondary c. tertiary d. quaternary e. globular
Which of the following produces ATP via the electron transport chain?
A. aerobic respiration B. anaerobic respiratio C. fermentatio D. glycolysis E. Photosynthesis A. A, B and C B. C, D and E C. A, B and E D. A, B, and D E. A, B, C, and E
Which of the following is NOT a characteristic of both DNA and RNA polymerases?
A) directionality of synthesis B) energy is provided by pyrophosphate C) hydrogen bonding of complementary nucleotides D) requirement for an initiation signal E) requirement for a primer