Which of the following PCR procedures includes all of the others?

A. DNA is reannealed at 50oC
B. DNA is denatured at 95oC.
C. Primers are extended at 72oC.
D. DNA is amplified for one cycle.


Answer: D

Biology & Microbiology

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The final step in a Sanger DNA sequencing reaction is to run the DNA fragments on a gel. What purpose does this serve?

A) It adds ddNTP to the end of each DNA fragment. B) It changes the length of the DNA fragments. C) It separates DNA fragments based on their charge. D) It separates DNA fragments generated during the sequencing reaction based on one-nucleotide differences in their size.

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The DNA site to which the repressor protein binds is the

A. operon. B. regulon. C. operator. D. repressor

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Inbreeding is due primarily to

a. migration. b. non-random mating. c. mutation. d. natural selection. e. adaptive radiation.

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An electrocardiogram (ECG, EKG) is a measure of the heart's:

a. electrical system. b. blood flow to the body. c. contraction rate. d. blood flow to heart muscle. e. pumping ability.

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