Which of the following is true about restriction endonucleases?
A. They make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.
B. Some make a blunt cut on the two DNA strands so that there are no single-strand regions and some make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.
C. They make a blunt cut on the two DNA strands so that there are no single-strand regions.
D. Depending on the incubation conditions, the same enzyme can either make a blunt cut on the two DNA strands so that there are no single-strand regions OR make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.
Answer: B
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