You have the following DNA template and want to use PCR to amplify the double-stranded region that is underlined. Select the primers that should be used.5' - CCGGATCAATCAATGCCGAATTTCCGTATAGGGCCTAGTAG - 3'3' - GGCCTAGTTAGTTACGGCTTAAAGGCATATCCCGGATCATC - 5'

A. 5' - AGGGC- 3' and 5' -GATTG- 3'.
B. 5' -GTTAG -3' and 5' -ATCCC -3'.
C. 3' -CAATC- 5'  and 3' -GCCCT- 5'.
D. 5' -CAATC- 3' and 5' -GCCCT- 3'.
E. 3' - AGGGC- 5' and 3' -GATTG- 5'.


Answer: D

Biology & Microbiology

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