What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with a recombinant DNA molecule.
II Cut the plasmid DNA using restriction enzymes (endonucleases).
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to non plasmid DNA fragments.
V. Use ligase to seal plasmid DNA to non plasmid DNA.
A) II, III, V, IV, I
B) III, II, IV, V, I
C) III, IV, V, I, II
D) IV, V, I, II, III
B
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Many of the different types of GPCRs in humans function to ____
a. distinguish different volatile molecules for odor recognition b. regulate metabolism by sensing and responding to changes in glucose levels c. regulate liver function by responding to different macromolecules d. aid in memory by binding to neurotransmitters in the amygdala e. trigger the fight of flight response by binding to hormones in the hypothalamus
Prokaryotes differ from eukaryotes in that they
a. have flagella. b. lack DNA. c. have chloroplasts and mitochondria. d. lack a nuclear envelope. e. have cell walls.
The result of “insulin shock” is ____
a. no insulin production b. type 2 diabetes c. hypoglycemia d. elevated blood glucose e. sudden heart failure
The term "reducing atmosphere" for the early Earth means that the atmosphere
A. contained little or no carbon dioxide. B. contained little or no free nitrogen. C. was much thinner around the surface of the Earth than now. D. contained only two or three kinds of gases. E. contained little or no free oxygen.