You wish to determine the cellular location of a protein but unfortunately it is not particularly antigenic (i.e., it can't develop a strong antibody response). How could you solve this problem?
A. Create a fusion protein with an epitope tag that can be visualized by incubation with fluorescently tagged antibody to the epitope.
B. Create a fusion protein with an epitope tag that is fluorescent without the addition of antibodies.
C. Create a fusion with green fluorescent protein (GFP). The fusion protein will fluoresce when streptavidin is added.
D. Express the protein as a fusion with biotin. The addition of GFP will cause fluorescence.
E. None of these methods will work.
A
You might also like to view...
Human female germ cells form
a. before birth. b. shortly after birth. c. at puberty. d. during late teenage-hood. e. at fertilization
The ability to live in a semi-submerged state, have a high salinity tolerance, and possess hollow tubes for root oxygenation best describes the dominant eastern North American tidal plant
A) bald cypress (Taxodium distichum). B) bayberry (Myrica pensylvanica). C) cordgrass (Spartina alterniflora). D) rockweed (Fucus spp.).
An intravenous (IV) solution must be __________________ to a person's red blood cells to prevent injury to the cells.
A. isotonic B. hypertonic C. hypotonic D. osmotic
Plants use what as a source of energy?
a. minerals b. fats c. oxygen d. vitamins e. light