You wish to determine the cellular location of a protein but unfortunately it is not particularly antigenic (i.e., it can't develop a strong antibody response). How could you solve this problem?

A. Create a fusion protein with an epitope tag that can be visualized by incubation with fluorescently tagged antibody to the epitope.
B. Create a fusion protein with an epitope tag that is fluorescent without the addition of antibodies.
C. Create a fusion with green fluorescent protein (GFP). The fusion protein will fluoresce when streptavidin is added.
D. Express the protein as a fusion with biotin. The addition of GFP will cause fluorescence.
E. None of these methods will work.


A

Biology & Microbiology

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