When processing a culture swab in the diagnostic laboratory the microbiologist should:

a. inoculate the media prior to making the direct smear.
b. roll the swab back and forth on the slide making sure to transfer organisms.
c. prepare the smear by rubbing the slide with one side of the swab and utilize the opposite slide to inoculate media.
d. cancel the direct Gram stain and only set up the media. Request a second swab for the direct smear.


C
With a single swab specimen, it is important for the microbiologist to carefully utilize one side of the swab to make the direct smear and the other side to inoculate the primary media to prevent contamination of the growth media.

Biology & Microbiology

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