Identify the incorrect statement regarding flow cytometry
a. Samples must be incubated with fluorescent molecules (such as fluorescent antibodies) before analysis.
b. It is possible to label samples with two fluorescent tags and determine whether cells are negative or positive for either one tag or the other, or both.
c. A one-dimensional histogram measures the amount of fluorescence versus cell number.
d. Samples must consist of a single cell type and not be composed of multiple cell types.
e. A laser is used to detect labeled cells.
f. A stream of cells in single file is generated by a nozzle.
D
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