The value of indirect Gram staining from a broth culture in comparison to a agar plate culture is:
a. there is no significant difference in the results of the indirect smear technique.
b. organisms tend to demonstrate natural cell shape and arrangement in liquid medium.
c. organisms tend to demonstrate more growth and easier visualization from agar plates.
d. none of the answers are correct.
B
Organisms that are grown in broth culture will demonstrate their natural shape and arrangement on indirect staining. Organisms smeared from agar plates tend to all clump together if the smear is not thin enough resulting in false presentation of cellular arrangements and over calling of or-ganisms in clusters.
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