How do the strands separate during PCR?

a. The primers separate the strands during the annealing step.
b. The DNA polymerase breaks the hydrogen bonds between the two strands.
c. The cycling of the temperatures breaks the hydrogen bonds between the two strands.
d. The high heat of the denaturation step breaks the hydrogen bonds between the two strands.


Answer: d. The high heat of the denaturation step breaks the hydrogen bonds between the two strands.

Biology & Microbiology

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