Differentiate between the various biosafety levels and when they are most appropriately used
What will be an ideal response?
Answer: Biosafety level 1 (BSL-1) is often used in teaching laboratories that do not work with known pathogens. In this case, barrier protection (gloves, coat) is recommended for use. Biosafety level 2 (BSL-2) is appropriate for a laboratory that works with moderate-risk pathogens. Barrier protection must be used, and manipulations that may generate aerosols must be performed in a biological safety cabinet. Biosafety level 3 (BSL-3) is appropriate for a laboratory that works with emerging and high-risk pathogens; barrier protection must be used. The laboratory is also negatively pressurized and fitted with filters to prevent pathogen escape. A biological safety cabinet is also in place for pathogen manipulations. Biosafety level 4 (BSL-4) is appropriate for a laboratory that works with emerging and high-risk pathogens, especially those spread by aerosols, for which there is no treatment, cure, or vaccine; barrier protection must be worn. Manipulations in a biosafety level 4 laboratory must be performed in a sealed biological safety cabinet or by personnel wearing positive pressure suits fitted with an air supply.
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