The three basic steps of polymerase chain reaction (PCR) are denaturation, primer annealing,

and primer extension. Which specification listed below is correct?

A) Primer extension—37° C when Taq polymerase is used
B) Primer design—oligonucleotide of 15-30 base pairs
C) Primer extension—10 minutes during each cycle
D) Primer annealing—95° C for 1 minute
E) Denaturation—50° C for 5 minutes


B

Health & Biomechanics

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A client with glaucoma is placed on a miotic drug and asks the nurse how his medication will work. How does the nurse respond?

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Identify and define the root in the term necrosis. 

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