A mutant you made in the laboratory with 5-bromouracil suddenly regains the wild-type phenotype. You discuss this phenomenon with your advisor and colleagues, and they suggest you try transposon mutagenesis to avoid this problem and create stable mutants

Why would mutants created with 5-bromouracil be more likely to regain the wild-type phenotype than mutants created via transposon mutagenesis?
What will be an ideal response?


Answer: Because a point mutation involves only a single base pair change, reversion is quite common relative to fixing an insertion of several nucleotides. Also common in Bacteria are suppressor mutations, which suppress the initial mutation and restore the wild-type phenotype (but not genotype) of a point mutation. However no analogous mechanism works to fix large-scale insertions or deletions.

Biology & Microbiology

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