Explain how T7 promoters regulate transcription and why cloning vectors contain them

What will be an ideal response?


Answer: The bacteriophage T7 polymerase gene is typically cloned on a vector such that it is under regulation of the lac promoter. In this way, analogs of lactose such as IPTG can be exogenously added to the growth medium at any given time to induce expression of the T7 polymerase. The T7 polymerase will recognize only T7 promoters, so only genes downstream to them are turned on in response to IPTG. Many cloning vectors contain T7 promoters upstream of the insert site so that their transcription can be easily manipulated.

Biology & Microbiology

You might also like to view...

Dr. Ratard was trying to determine the cause of a mysterious epidemic affecting fish in the gulf of New Mexico. His proposal that the deaths were caused by an organism called a protist is considered a(n) _________

A. theory. B. data set. C. hypothesis. D. conclusion. E. experiment.

Biology & Microbiology

A frequent complication of scalded skin syndrome is a secondary infection caused by

A. M. luteus. B. S. pyogenes. C. S. epidermidis. D. Pseudomonas spp.

Biology & Microbiology

What is the feature of horsetails that was useful before the existence of scouring pads?

a. rhizomes b. silica in the stems c. photosynthetic cells d. cones at the tips e. scale-like leaves

Biology & Microbiology

How does a cell "inform" the immune system that it is infected with a virus?

A) It releases histamine into the bloodstream. B) It displays viral proteins on its membrane. C) It synthesizes enzymes that activate helper T cells. D) It secretes steroid hormones. E) It produces interferon.

Biology & Microbiology