A new prokaryote is recovered from a remote saline hot spring. Design a set of experiments to determine whether the organism is thermophilic or thermoduric, halophilic or halotolerant

What will be an ideal response?


The experimental design should include a control in which the organism is grown in the salt conditions and at the temperature it was recovered from. One set of samples should be cultured at the same salt concentration but varying the temperatures. Another set of samples should be cultured at the temperature of the hot spring but varying the salt concentration. One culture should be grown at the lowest temperature and salt concentration used in the two experimental sets. Bacterial growth is most reliably measured using the viable plate count method (assuming the organism can be grown on plates). Alternatively, bacterial numbers may be determined using a cell counter. An excellent answer will describe the organism which can be expected to grow under the various conditions (thermophilic halophile, thermophilic halotolerant, thermoduric halophile, and so forth).
Bloom's Taxonomy: Synthesis
Section: Growth Requirements
Learning Outcome: 6.6

Biology & Microbiology

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Compare and contrast the works of Louis Pasteur and Robert Koch in terms of both applied and basic science

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Biology & Microbiology

Different aminoacyl-tRNA synthetases:

A. may bind the same amino acid. B. may bind the same tRNA. C. do not appear to recognize the same features of tRNAs. D. will always recognize both the acceptor stem and anticodon sequences of tRNAs. E. will always recognize the complete tRNA anticodon sequence.

Biology & Microbiology

Both honeybees and birds have wings for flight; thus, these structures are homologous in the

two animal groups.

a. true, because the wings have a similar function and thus must be anatomically similar b. false, because the wings look very different between the two groups c. true, because all animals have a common ancestor d. false, because birds and honeybees do not share a common ancestor from which this trait was derived

Biology & Microbiology

During chordate evolution, what is the sequence (from earliest to most recent) in which the following structures arose? 1. amniotic egg 2. paired fins 3. jaws 4. swim bladder 5. four-chambered heart

A) 2, 3, 4, 1, 5 B) 3, 2, 4, 5, 1 C) 3, 2, 1, 4, 5 D) 2, 1, 5, 3, 4

Biology & Microbiology