A PCR was designed to clone a DNA sequence of 1.5 kb. When the products were run on an agarose gel, there was a smear of bands ranging from 1.5 kb to 6 kb. Which of the following is a reason for this result?

A) the thermocycler malfunctioned B) one of the primers did not bind
C) the polymerase overran the reverse primer D) the polymerase denatured


Answer: B

Biology & Microbiology

You might also like to view...

The lowermost and outermost whorl on a floral shoot consists of petals

____________________ Indicate whether the statement is true or false.

Biology & Microbiology

What type of bond is formed when two atoms share electrons?

A.) electronic bond B.) covalent C.) electrostatic D.) hydrogen E.) ionic

Biology & Microbiology

The diagram shows the rate of diffusion into a cell using carrier proteins.Carrier proteins are saturated at which point?

A. 1 B. 2 C. 3 D. 4

Biology & Microbiology

Which of the following describe source-sink metapopulations? (Check all that apply.)A) Sink populations near source populations are less likely to go extinct.B) Sink populations without access to immigrants from source populations are less likely to go extinct.C) Source populations usually occupy better habitats.D) Source populations are less likely to go extinct.E) The emigration rate from source populations exceeds that from sink populations.

What will be an ideal response?

Biology & Microbiology