Which of the following is not true of oligonucleotide-directed mutagenesis?
A. Two complementary oligonucleotides, each identical in sequence to the cloned gene, are annealed to the opposite strands of the cloned gene.
B. Two complementary oligonucleotides, each with the desired base change, are annealed to the opposite strands of the cloned gene.
C. After repeated cycles of PCR, the mutation containing DNA predominates in the population.
D. Nonmutant template plasmid DNA can be selectively eliminated by cleavage of the methylated template strand.
E. The two annealed mutant DNA strands serve as primers of DNA synthesis in both directions around the plasmid vectors.
A
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Would you expect dialysis membrane to be permeable to egg albumin, a protein with molecular weight of approximately 36,800? Why or why not?
What will be an ideal response?
Which of the following is a characteristic of Nanos protein?
A. Throughout the first seven nuclear divisions, Nanos concentration is higher in the anterior pole than in the posterior pole. B. Nanos represses Hunchback in the posterior pole. C. Nanos is a transcriptional activator of many segmentation genes. D. Nanos represses Caudal in the anterior pole.
Which type of vaccine has been used in place of inactivated whole cell vaccine?
A. Acellular subunit B. Whole cell attenuated C. Adjuvant D. Toxin
What type of reasoning did the pediatrician use to generate her prediction about Luke’s future growth in height?
A. Inductive reasoning
B. Applied theory
C. Deductive reasoning
D. Reductionism
Clarify Question
1. What is the key concept addressed by the question?
2. What type of thinking is required?
3. What key words does the question contain and what do they mean?
Gather Content
1. What do you know about different types of reasoning?How does it relate to the question?
Consider Possibilities
1. What other information is related to the question? Which information is most useful?
Choose Answer
1. •Given what you now know, what information and/or problem solving approach is most likely to
produce the correct answer?
Reflect on Process
1. Did your problem-solving process lead you to the correct answer? If not, where did the process break
down or lead you astray? How can you revise your approach to produce a more desirable result?