Which of the following is the correct sequence of PCR?

A) Heat to 90°C (to separate DNA), then cool to 50°C (to bind primers and enzymes), and then reheat to 70°C (to synthesize DNA).
B) Heat to 90°C (to bind primers and enzymes), then cool to 50°C (to separate DNA), and then reheat to 70°C (to synthesize DNA).
C) Heat to 70°C (to bind primers and enzymes), then cool to 50°C (to synthesize DNA), and then reheat to 90°C (to separate DNA).
D) Heat to 70°C (to separate DNA), then cool to 50°C (to bind primers and enzymes), and then reheat to 90°C (to synthesize DNA).


A

Biology & Microbiology

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