What are the key replication enzymes at the replisome, and how is DNA replication on both leading and lagging strands made efficient through the conformation of the DNA at the replisome in prokaryotes?

Question

What will be an ideal response?

Answer 1

The key replication enzymes at the replisome are helicase, primase, and DNA polymerase III. To make replication more efficient, the lagging-strand DNA is folded so that its DNA polymerase III is complexed with the DNA polymerase III on the leading strand (forming the DNA Pol III holoenzyme). The folding of the lagging-strand template also makes production of sequential Okazaki fragments more efficient by bringing the 3' end of each completed Okazaki fragments near the site where the next Okazaki fragment will start.