Describe the technique of fluorescence resonant energy transfer (FRET) and its purpose

What will be an ideal response?


Ans: FRET is a type of real-time PCR that can be used to quantify the amount of amplicon product formed at any time during the reaction. It relies on two fluorophores, a "reporter" and a "quencher." One of each is attached to each of the two probes that are specific for the correct PCR product. When the correct product is formed, the two are in close proximity, and reporter excites the quencher to fluoresce. When the correct product is not formed, the two remain separate and only the reporter will fluoresce.

Biology & Microbiology

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Mobile genetic elements that encode proteins that contribute to or confer virulence are known as contiguous sequences.

Answer the following statement true (T) or false (F)

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What is the relationship between operators, transcription units, and operons?

a. Operators consist of transcription units and operons. b. Operons consist of transcription units and operators. c. Operators consist of multiple transcription units associated with a single operon. d. Operons consist of multiple operators associated with a single transcription unit. e. Operons consist of multiple transcription units associated with a single operator.

Biology & Microbiology

The term used to describe the fact that cancer cells have lost the specializations of the cells from which they descend is

A) heritable. B) angiogenic. C) oncogenic. D) dedifferentiated.

Biology & Microbiology

Filtrate moves from the space within the glomerular capsule to ________, the portion of the renal tubule with microvilli

Fill in the blank(s) with correct word

Biology & Microbiology